1. 队名：Great Bay China
项目名称：microbial Compartmentalization AssisTed Nepetalactol Ingredient Production
（1）Grand Prize Winner
（5）Best New Composite Part
（6）Best Part Collection
（7）Best Product Design
（8）Best New Basic Part
Nepetalactone is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE)stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.
项目名称：SPIDERMAN_SPIDroin EngineeRing with chroMoprotein And Natural dye
（1）Grand Prize Winner;
（2）Best Education and Public Engagement;
（4）Best Integrated Human Practices;
（7）Best New Basic Part;
（8）Best New Composite Part;
（9）Best Part Collection;
Spider silk serves as a new material with superior properties that can be applied in medication, cloth, and aerospace fields. However, spider breeding is not applicable due to spider's fierce behavior. The current approach is to produce recombinant spidroins (silk proteins) from other chassis and spin them into silk. This year , we aim to manufacture recombinant spider silk with E.coli and color the silk for application in cloth industry. We modularized three significant domains of spidroin - the N-terminal, the repetitive region, and the C-terminal - and integrated them into various spidroin to form silk. We then dyed the silk with microbial natural pigments deoxyviolacein and indigo. To obtain better color and a more convenient dying process, we fused the repetitive region to chromoproteins and mixed them with spidroin during spinning. Our team hopes to provide a novel approach for cloth production and explore new possibilities for spider silk applications.
项目名称：Fresh Air, the clever heir
（1）Best Education and Public Engagement;
（2）Best Integrated Human Practices;
（4）Best New Basic Part;
（5）Best New Composite Part;
（6）Best Part Collection;
（8）Best Supporting Entrepreneurship;
To increase our algae system's efficiency of reducing indoor pollutants such as CO2 and VOCs, we applied two enzymes, carbonic anhydrase(CA) and cytochrome P450 2E1(CYP2E1) to improve algal absorption of CO2 and VOCs, respectively. We produced enzymes by engineering Bacillus subtilis 168 with BioBricks we created and then finally put them into our system. We also did some experiments to analyze the activity of our enzymes and test whether our system is efficient enough to refresh indoor air.
项目名称：Recombinase-Based Biological Relay devices (RBBR),
（3）Best New Basic Part;
（4）Best Part Collection;Best Wiki
Generally, the relay, the key component of electrical automatic control system, receives the output signal of a control module and thus shifts the ON/OFF state of a separated working module. Based on the recombinase-attB/attP system and unidirectional terminator, we constructed a set of orthogonal Recombinase-Based Biological Relay devices (RBBR), whose response intervals were characterized by accurate quantifying method so that they can be predictably adapted to different genetic circuits. We designed and constructed a resolution extensible analog-digital converter (ADC), which converts the consecutive analog quantities (the strength of an inducible promoter) into discrete digital signals (indicated by different chromoproteins), allowing the digitized processing and storage of signals. Beyond the common use of recombinase system as simple response to two input levels, our project achieves modifying and utilizing the response interval of this system. The application of relay in genetic circuit can contribute to the improvement of the modularity of artificial biological system.
项目名称：ENGINEERING A CELL-FREE HEXAVALENT CHROMIUM DETECTION SYSTEM
奖项：Best Education and Public Engagement
Methylophaga flavin-containing monooxygenase (bFMO) is the gene responsible for the conversion of the Indole into Isatin, which is then catalyzed into indigoid compounds. These compounds display an indigo color which can be easily detected (KAIST). Our project will focus on using bFMO as indicator to determine when toxic hexavalent chromium is present. The bFMO will be cloned into the C4-HSL circuit created by our team last year, in order to show the indigo color can be produced and easily seen. This will be done by replacing GFPa1 (green fluorescent protein) with the bFMO gene. Next, bFMO will be cloned into a construct with a T7 promoter, which we believe will be the most efficient promoter for bFMO. This construct will then be tested to ensure that the promoter will produce bFMO. Once this is done, the chrB repressor will be introduced. In order to determine when hexavalent chromium is present, a repressor, chrB, will be cloned into a construct along with the promoter, chrP, associated with the repressor. This construct will then be cloned into a pet11a backbone, which already has both the T7 promoter and rbs needed. The plasmid should then produce chrB, which will disrupt the chrP causing bFMO to not be produced. A commercial cell-free extract kit will then be used on this construct, and the T7-chrB plasmid will be added in order to make ChrB. When chromium is detected the ChrB repressor will be ‘knocked off,’ allowing the promoter to function and bFMO to catalyze into indigoid compounds, emitting a detectable indigo color.
奖项：Best New Composite Part
DNA is a biological macro-molecule which can carry huge amount of information accurately, and this feature can be used to achieve data recordings in vivo. Our project aims to build a biological recorder that can monitor extracellular information and record it on DNA. Recombinase or CRISPR base-editor is used to target specific DNA addresses and generate mutations in a reporter gene, so the recordings can be quantitatively measured to infer intensity and duration information about the chemicals of disease, such as inflammatory bowel disease. Additionally, we designed a hardware for in situ biomolecular detection to monitor gastrointestinal health. This platform could enable more precise detection and could help improve the management and diagnosis of gastrointestinal disease.
奖项：Best Part Collection
To create a well-functioning adhesive, cohesion--the ability to maintain the shape of the material--and adhesion--the ability to stick to underwater surfaces--should cooperate. Our toolbox consists of several groups of biological parts from marine creatures (mussel and barnacles) and prokaryotes (E.coli MG1655 and Marine Archaea). These proteins will provide cohesion, adhesion, and other special qualities. We will then combine these proteins using principles of synthetic biology to create protein adhesives with high-performance and biocompatibility. Their potentials are limitless and can be applied to many fields.
奖项：Best Part Collection
We consume fruits and vegetables every day without knowing whether or not agricultural residues (i.e. pesticides & heavy metals) are present. In 2017, according to the UN, exposure to pesticides caused 200,000 to 300,000 deaths annually. Additionally, lead exposure alone is responsible for a death rate of 25.3 per 100,000 individuals in East Asia. Current methods of agricultural residue detection are not easily accessible to the public and are inconvenient for everyday use. Thus, our project aims to allow for convenient visualization of agricultural residues by designing colored proteins that can directly interact with these residues. We envision a system where our designed proteins can be applied directly on food items to detect the presence of residues. Our final product can be used by consumers, distributors and farmers alike.
项目名称：Breakdown of Trimethylamine via Trimethylamine Dehydrogenase to Minimize Heart Disease Caused by Red Meat Consumption
奖项：Best Plant Synthetic Biology
The consumption of red meat has been linked to atherosclerosis, a form of heart disease caused by the buildup of plaque in the arteries. Recently, it was discovered that the combination of choline and L-carnitine from red meat, are converted to trimethylamine (TMA) in the body. TMA is a precursor to trimethylamine N-oxide (TMAO), which is found to exacerbate cholesterol buildup, ultimately leading to atherosclerosis. In this study, we targeted this pathway by breaking down the precursor, TMA, before TMAO is formed. A system designed to concurrently degrade TMA and subdue its toxic by-product, formaldehyde, was implemented in E. coli for these purposes. This system is composed of Trimethylamine Dehydrogenase (TMADH) and Formaldehyde Dehydrogenase (FDH) which are being tested independently for their degradation properties. Our approach has proven that TMADH is effective in TMA degradation and FDH can be expressed in a bacterial vector to minimize the presence of formaldehyde.