OCR A Level Biology:复习笔记1.2.14 Serial Dilutions

Serial Dilutions

  • Serial dilutions are created by taking a series of dilutions of a stock solution. The concentration decreases by the same quantity between each test tube
    • They can either be ‘doubling dilutions’ (where the concentration is halved between each test tube) or a desired range (e.g. 0, 2, 4, 6, 8, 10 mmol dm-3)

     

  • Serial dilutions are completed to create a standard to compare unknown concentrations against
    • The comparison can be:
      • Visual
      • Measured through a calibration/standard curve
      • Measured using a colourimeter

       

    • They can be used when:
      • Counting bacteria or yeast populations
      • Determining unknown glucose, starch, protein concentrations

       

     

Making serial dilutions

 

 

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