IB DP Biology: SL复习笔记2.6.2 Translation

Translation

  • Translation is the synthesis of polypeptides on ribosomes
  • This stage of protein synthesis occurs in the cytoplasm of the cell
  • After leaving the nucleus, the mRNA molecule attaches to a ribosome
  • A ribosome is a complex structure that is made of a large and small subunit
    • Ribosomes are themselves made of proteins and RNA (called ribosomal RNA or rRNA)
  • There are binding sites on the subunits for the various other molecules involved in translation

Cell-components_Ribosome-1

A ribosome is built of large and small subunits, ribosomal RNA and an area on the surface that catalyses the formation of peptide bonds in a newly-synthesised protein

Exam Tip

Make sure you learn both stages of protein synthesis fully. Don’t forget WHERE these reactions take place – transcription occurs in the nucleus but translation occurs in the cytoplasm!

Genetic Code & mRNA

  • The amino acid sequence of polypeptides is determined by mRNA according to the genetic code
  • mRNA varies in length, depending on the length of the gene, but is around 2,000 nucleotides long, on average (in mammals)
  • Only certain genes are transcribed in a particular cell, depending on the function of that cell
    • The gene for rhodopsin (a light-sensitive protein in the eye) is transcribed to mRNA in retina cells, but not transcribed in other body cells where rhodopsin is not required; that would be a waste of cellular energy

Exam Tip

Most RNA exists as mRNA but don't forget the other types; transfer RNA (tRNA) and ribosomal RNA (rRNA).

Codons

  • Codons of three bases on mRNA correspond to one amino acid in a polypeptide
  • The four nucleotide bases in mRNA are not enough to code for 20 separate amino acids
  • Pairs of nucleotides would only give 16 combinations (42 = 16), which is still not enough
  • Triplets of nucleotides would yield 64 combinations (43 = 64), which is more than enough
  • Different triplets code for the same amino acid, giving some protection against mutation
    • A triplet is a sequence of three DNA bases that codes for a specific amino acid
    • A codon is a sequence of three mRNA bases that codes for a specific amino acid
    • A codon is transcribed from the triplet and is complementary to it
  • An anticodon is a sequence of three transfer RNA (tRNA) bases that are complementary to a codon
    • The transfer RNA carries the appropriate amino acid to the ribosome
    • The amino acid can then be condensed onto the growing polypeptide chain
  • Certain codons carry the command to stop translation when the polypeptide chain is complete ('Stop codons')

mRNA Codons and Amino Acids Table

mRNA-Codons-and-Amino-Acids-Table

Worked Example

Use the rules of base-pairing and the mRNA Codons and Amino Acids Table (above) to deduce the amino acid sequence coded for by the following DNA sense strand sequenceTTC GAG CAT TAC GCC

Step 1: Work out the antisense sequence using A-T and C-G base pairing rules

AAG CTC GTA ATG CGG

Step 2: Work out the mRNA codons, complementary to the antisense strand

UUC GAG CAU UAC GCC

Step 3: Use the mRNA Codons and Amino Acids Table (above) to work out the first amino acid

First base in codon = U, second base = U, third base = C

So we're looking in the top-left box of the table; this amino acid is Phe

Step 4: Repeat for the remaining 4 codons

GAG = Glu

CAU = His

UAC = Tyr

GCC = Ala

Answer: The final sequence of amino acids is Phe-Glu-His-Tyr-Ala

Codons & Anticodons

  • Translation depends on complementary base pairing between codons on mRNA and anticodons on tRNA
  • In the cytoplasm, there are free molecules of tRNA (transfer RNA)
  • The tRNA molecules bind with their specific amino acids (also in the cytoplasm) and bring them to the mRNA molecule on the ribosome
  • The triplet of bases (anticodon) on each tRNA molecule pairs with a complementary triplet (codon) on the mRNA molecule
  • Two tRNA molecules fit onto the ribosome at any one time, bringing the amino acid they are each carrying side by side
  • A peptide bond is then formed (by condensation) between the two amino acids
    • The formation of a peptide bond between amino acids is an anabolic reaction
    • It requires energy, in the form of ATP
    • The ATP needed for translation is provided by the mitochondria within the cell
  • This process continues until a ‘stop’ codon on the mRNA molecule is reached – this acts as a signal for translation to stop and at this point the amino acid chain coded for by the mRNA molecule is complete
  • This amino acid chain then diffuses away from the ribosome and forms the final polypeptide

tRNA-molecules-bind-with-amino-acids

The translation stage of protein synthesis – tRNA molecules bind with their specific amino acids

Process-of-Translation-1Process-of-Translation-2

The translation stage of protein synthesis – an amino acid chain is formed

How-ribosomes-build-proteins

An polypeptide forms as peptide bonds are added in sequence

 

 

 

 

 

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